Biovalorization of Lignocellulosic Materials for Xylitol Production by the Yeast Komagataella pastoris

The main goal of this study was to screen different lignocellulosic materials for their ability support the cell growth yeast Komagataella pastoris and production xylitol. Several materials, namely banana peels, brewer’s spent grains (BSGs), corncobs, grape pomace, stalks, sawdust, were subjected dilute acid hydrolysis obtain sugar rich solutions that tested as feedstocks cultivation K. pastoris. Although culture able grow in all hydrolysates, a higher biomass concentration obtained peels (15.18 ± 0.33 g/L) stalks (14.58 0.19 g/L), while highest xylitol (1.51 0.07 reached BSG hydrolysate with yield 0.66 0.39 g/g. Cell from improved by detoxifying using activated charcoal, resulting fourfold increase production, 3.97 0.10 g/L. Moreover, concomitant arabinose consumption, arabitol synthesis noticed, reaching maximum 0.82 0.05 g/L on 0.60 0.11 These results demonstrate feasibility waste, especially BSG, feedstock coproduction arabitol. Additionally, it demonstrates use suitable microorganism integrate zero-waste biorefinery, transforming waste into two high-value specialty chemicals high market demand.